Amazing stuff! Discovering the secrets of life!
"... has developed a new RNA detection method named BOLORAMIS (short for “Barcoded Oligonucleotides Ligated On RNA Amplified for Multiplexed and parallel In Situ analyses”) that overcomes this problem. BOLORAMIS enables the design and uses a new type of DNA probe that directly binds its RNA target and allows the straight-forward synthesis of a barcoded DNA amplicon, which can be visualized by fluorescent in situ hybridization (FISH) or sequenced in situ. BOLORAMIS enables the analysis of different classes of RNAs with higher specificity and sensitivity than FISSEQ and other methods, works in the context of cells and tissues, and can be highly multiplexed. ...
“We used a co-culture system of neuronal cells and brain microglia which are known to interact in many normal and disease processes, and targeted 96 different messenger RNAs simultaneously,” ... “This allowed us to uncover the spatial relationships between specific cells and RNAs.” ...
“In future research, BOLORAMIS’s high functionality in complex human tissues and human-specific organoids may well give us an edge in deciphering RNA signatures related to neurological disorders,” ...
With BOLORAMIS we have solved some of the challenges that technologies in the spatial transcriptomics field are facing. It gives us a significant advantage for understanding the behavior of molecular networks in normal and pathological processes, and for investigating new drug targets, and developing clinical diagnostics in the native context of tissues ..."
“We used a co-culture system of neuronal cells and brain microglia which are known to interact in many normal and disease processes, and targeted 96 different messenger RNAs simultaneously,” ... “This allowed us to uncover the spatial relationships between specific cells and RNAs.” ...
“In future research, BOLORAMIS’s high functionality in complex human tissues and human-specific organoids may well give us an edge in deciphering RNA signatures related to neurological disorders,” ...
With BOLORAMIS we have solved some of the challenges that technologies in the spatial transcriptomics field are facing. It gives us a significant advantage for understanding the behavior of molecular networks in normal and pathological processes, and for investigating new drug targets, and developing clinical diagnostics in the native context of tissues ..."
"We present barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel insitu analyses (BOLORAMIS), a reverse transcription-free method for spatially-resolved, targeted, in situ RNA identification of single or multiple targets. ..."
Here is the link to the underlying paper:
Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses (with guest access)
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