Amazing stuff!
"... Researchers from the University of Georgia have now developed an improved light-sheet microscope with the ability to perform fast 3D imaging of zebrafish seizures. ...
the researchers found that seizures originated in the hindbrain and propagated forward (anteriorly). This corroborates a previous study examining 3D imaging of zebrafish seizures, but contradicts two earlier studies of 2D imaging (reported in Frontiers in Neural Circuits and eNeuro) that observed propagation in the reverse direction. ..."
From the abstract:
"Light sheet microscopy is a powerful tool for imaging live organisms. To enable high-speed volumetric imaging, we have developed a light sheet system incorporating an electrically tunable lens (ETL) capable of capturing volumes up to 499 × 499 × 150 μm3 at 4 volumes per second with near diffraction-limited resolution. The system employs sensorless adaptive optics to correct ETL induced system aberrations, extending the usable field of view by fivefold.
We apply this system to image the propagation of seizures in zebrafish larvae and observe that seizures originate in the posterior brain, propagate anteriorly toward the optic tectum and gradually subside over tens of seconds."
UGA study is among the first to provide a high-resolution 3D video of a seizure event (original news release)
Fast volumetric imaging of a zebrafish seizure model with adaptive optics light sheet microscopy (open access)
Mid-seizure activity Scientists capture a snapshot of a larval zebrafish brain during a seizure. White and red mark regions with the greatest neuronal activity, while blue tones indicate lower activity.
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