Amazing stuff!
"... So researchers designed a moving-picture system [M25] that can mount to the side port of a standard commercial microscope: an array of 25 miniature cameras, each capturing the same image simultaneously but at different depths. A specially designed, multi-focus grating made of nanometer-etched glass splits the light evenly into each camera, and more gratings in front of each lens correct any oddities ...
To test M25, researchers imaged live model organisms like a nematode and fly and oyster parasite larvae, finding that it could capture organisms up to 50 micrometers deep. Importantly, the microscope could capture fluorescent and non-fluorescent samples, so it doesn’t require invasive marking techniques that can damage samples. In the video above, the cameras capture a wormy nematode subject wriggling and writhing. ..."
From the abstract:
"High-speed volumetric imaging of whole-organism dynamics is often constrained by trade-offs between speed, resolution, and imaging depth. We present the M25 microscope, a 25-camera-array, aberration-corrected refocusing multifocus imaging system that captures 3D volumes simultaneously across 25 focal planes using a synchronized array of machine-vision cameras. Each camera incorporates a custom-blazed grating to correct chromatic dispersion, enabling a simplified, sensitive, and scalable multifocus setup for large fields of view while maintaining high spatial resolution across the imaging volume.
M25 achieves imaging speeds of volumes per second over imaging volumes of µ. This method enables both noninvasive, label-free brightfield and highly sensitive fluorescence imaging.
We demonstrate its capabilities in 3D particle tracking, fluorescent and brightfield imaging of D. melanogaster larval dynamics, and C. elegans locomotion and neural activity. This method enables fast and sensitive 3D imaging for biological studies and has potential applications across a broad range of diffractive imaging modalities."
Multifocus microscope pushes the limits of fast live 3D biological imaging "Using 25 cameras to capture detailed dynamics, the new system extends classical multifocus microscopy to study development, locomotion, and neuroscience in real time"
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