Amazing stuff!
The study of the most famous research insect of the world (besides the bee, ant, and the mosquito) keeps on giving! We should actually erect a large statue for this insect! 😄
"Animal development can progress quite rapidly, with cellular lineages proliferating and differentiation status changing minute to minute. Calderon et al. have now visualized development in the fruit fly Drosophila in greater detail than ever before. Taking advantage of the ability to produce collections of Drosophila embryos that differ in developmental stage by only seconds or minutes, the authors have analyzed, on a single-cell basis, how chromatin accessibility and gene expression shift during Drosophila embryogenesis. This single-cell atlas of Drosophila embryogenesis reveals cell lineages and their developmental relationships and links enhancer usage and gene expression."
From the abstract:
"... We profiled chromatin accessibility in almost 1 million nuclei and gene expression in half a million nuclei from eleven overlapping windows spanning the entirety of embryogenesis (0 to 20 hours). To exploit the developmental asynchronicity of embryos from each collection window, we applied deep neural network–based predictive modeling to more-precisely predict the developmental age of each nucleus within the dataset, resulting in continuous, multimodal views of molecular and cellular transitions in absolute time. With these data, the dynamics of enhancer usage and gene expression can be explored within and across lineages at the scale of minutes, including for precise transitions like zygotic genome activation.
CONCLUSION
This Drosophila embryonic atlas broadly informs the orchestration of cellular states during the most dynamic stages in the life cycle of metazoan organisms. The inclusion of predicted nuclear ages will facilitate the exploration of the precise time points at which genes become active in distinct tissues as well as how chromatin is remodeled across time."
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