Sunday, August 21, 2022

New mRNA cancer vaccine targets lymph nodes for stronger immune response

Good news! Cancer is history (soon)! Now that we have gained so much more experience with mRNA vaccines let's put them to good use!

"... Development on these therapies has greatly accelerated in recent years, thanks largely to the COVID-19 pandemic. The breakthrough is now opening up the possibility of mRNA therapies for a range of other diseases, including HIV, influenza, malaria, Lyme disease, herpes, shingles, or even to repair heart tissue after a heart attack. ...
scientists tweaked the recipe for the lipid nanoparticles that transport the mRNA. This causes different molecules from the bloodstream to gather on the surface of the particles, which in turn bind to receptors in different organs. After testing a few combinations of properties, the team created lipid nanoparticles that favored delivery to the lymph nodes over the liver by a three-to-one ratio. ...
In tests in mice with metastatic melanoma, the team found that the treatment significantly inhibited the tumors, when combined with another treatment called anti-PD-1 therapy. Better yet, complete remission occurred in 40% of cases, and the cancer didn’t recur down the track – even after the scientists later injected them with metastatic tumor cells. ..."

"Current messenger RNA (mRNA) vaccines in the clinic were reported to induce side effects in the liver, such as reversible hepatic damages and T cell–dominant immune-mediated hepatitis, which might be caused by the undesired expression of antigens in the liver. Therefore, exploring a lymphoid-organ–specific mRNA vaccine could be a promising strategy for developing next-generation mRNA vaccines. Herein, we reported a lymph-node–targeting mRNA vaccine based on lipid nanoparticles named 113-O12B for cancer immunotherapy. The targeted delivery of the mRNA vaccine elicits robust CD8+ T cell responses, exhibiting excellent protective and therapeutic effects on B16F10 melanoma. Notably, 113-O12B can efficiently deliver both a full-length protein and a short-peptide–based, antigens-encoded mRNA, thus providing a universal platform for mRNA vaccines."

From the abstract:
"The targeted delivery of messenger RNA (mRNA) to desired organs remains a great challenge for in vivo applications of mRNA technology. For mRNA vaccines, the targeted delivery to the lymph node (LN) is predicted to reduce side effects and increase the immune response. In this study, we explored an endogenously LN-targeting lipid nanoparticle (LNP) without the modification of any active targeting ligands for developing an mRNA cancer vaccine. The LNP named 113-O12B showed increased and specific expression in the LN compared with LNP formulated with ALC-0315, a synthetic lipid used in the COVID-19 vaccine Comirnaty. The targeted delivery of mRNA to the LN increased the CD8+ T cell response to the encoded full-length ovalbumin (OVA) model antigen. As a result, the protective and therapeutic effect of the OVA-encoding mRNA vaccine on the OVA-antigen–bearing B16F10 melanoma model was also improved. Moreover, 113-O12B encapsulated with TRP-2 peptide (TRP2180–188)–encoding mRNA also exhibited excellent tumor inhibition, with the complete response of 40% in the regular B16F10 tumor model when combined with anti–programmed death-1 (PD-1) therapy, revealing broad application of 113-O12B from protein to peptide antigens. All the treated mice showed long-term immune memory, hindering the occurrence of tumor metastatic nodules in the lung in the rechallenging experiments that followed. The enhanced antitumor efficacy of the LN-targeting LNP system shows great potential as a universal platform for the next generation of mRNA vaccines."

New mRNA cancer vaccine targets lymph nodes for stronger immune response

New Targeted Cancer Vaccines Eliminate Tumors and Prevent Recurrence in Mice Tufts researchers devise an mRNA-based cancer vaccine delivered directly into the lymphatic system, leading to a more potent response than other cancer vaccines

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