Friday, March 04, 2022

Researchers Use Ultrasound to Control Neurons in Mice

Amazing stuff!

"More than a decade ago, scientists developed optogenetics, a method to turn cells on and off with light. The technique allows scientists to spur or suppress cells' electrical activity with just the flip of a switch to tease apart the roles of specific cell types. But because light doesn’t penetrate deep into tissues, scientists need to surgically implant light sources to illuminate cells below the surface of the skin or skull.

In a new study published today (February 9) in Nature Communications, researchers report they’ve found a way to use ultrasound to noninvasively activate mouse neurons, both in culture and in the brains of living animals. The technique, which the authors call sonogenetics, elicits electrical activity in a subset of brain cells that have been genetically engineered to respond to sound waves. ..."

From the abstract:
"Ultrasound has been used to non-invasively manipulate neuronal functions in humans and other animals. However, this approach is limited as it has been challenging to target specific cells within the brain or body. Here, we identify human Transient Receptor Potential A1 (hsTRPA1) as a candidate that confers ultrasound sensitivity to mammalian cells. Ultrasound-evoked gating of hsTRPA1 specifically requires its N-terminal tip region and cholesterol interactions; and target cells with an intact actin cytoskeleton, revealing elements of the sonogenetic mechanism. Next, we use calcium imaging and electrophysiology to show that hsTRPA1 potentiates ultrasound-evoked responses in primary neurons. Furthermore, unilateral expression of hsTRPA1 in mouse layer V motor cortical neurons leads to c-fos expression and contralateral limb responses in response to ultrasound delivered through an intact skull. Collectively, we demonstrate that hsTRPA1-based sonogenetics can effectively manipulate neurons within the intact mammalian brain, a method that could be used across species."

Researchers Use Ultrasound to Control Neurons in Mice | The Scientist Magazine® A study shows “sonogenetics” could be a useful new tool in neuroscience and other fields.

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