Amazing stuff! Any bodily fluid can tell something! For tears, this is only the beginning.
"... We are interested in investigating extracellular vesicle-based multi-biomarkers from drops of tears for clinical diagnostics. Since the lacrimal glands secrete tears in the eyelids through filtration from blood plasma, which circulates the entire body by collecting exosomes from organs and tissues, it is time to recognize that tears contain rich clinical information about different parts of our body ...
exosomes ... play an important role in cell-to-cell communication and influence both physiological and pathological processes ...
The new system, dubbed “Incorporated Tear Exosomes Analysis via Rapid-isolation System,” or simply iTEARS for short, can separate exosomes from tear samples in just five minutes, as opposed to the hours or even days required by traditional approaches ...
a nanoporous membrane with oscillating pressure flow that effectively reduces clogging. The system allowed the researchers to tag proteins with fluorescent probes for tracking and later transfer to other instruments for further analysis, as well as extract nucleic acids for analysis. ...
In a demonstration, using iTEARS, the researchers were able to identify dry eye disease and diabetic retinopathy in samples taken from healthy controls and patients. But tears could be used to diagnose a range of diseases that affect the body, not just the eyes, the researchers claim. ..."
exosomes ... play an important role in cell-to-cell communication and influence both physiological and pathological processes ...
The new system, dubbed “Incorporated Tear Exosomes Analysis via Rapid-isolation System,” or simply iTEARS for short, can separate exosomes from tear samples in just five minutes, as opposed to the hours or even days required by traditional approaches ...
a nanoporous membrane with oscillating pressure flow that effectively reduces clogging. The system allowed the researchers to tag proteins with fluorescent probes for tracking and later transfer to other instruments for further analysis, as well as extract nucleic acids for analysis. ...
In a demonstration, using iTEARS, the researchers were able to identify dry eye disease and diabetic retinopathy in samples taken from healthy controls and patients. But tears could be used to diagnose a range of diseases that affect the body, not just the eyes, the researchers claim. ..."
From the abstract:
"Nanoscale small extracellular vesicles (sEVs, exosomes) in tears allow us to investigate the multisignatures of diseases. However, the translations of tear sEVs for biomarker discovery and clinical diagnostics are practically limited by low recovery, long processing time, and small sample volume. Here, we report an incorporated tear-exosomes analysis via rapid-isolation system (iTEARS) via nanotechnology to discover the secrets of ocular disorders and systemic diseases. We isolate exosomes rapidly with high yield and purity from a few teardrops (∼10 μL) within 5 min via nanoporous membrane-based resonators for the quantitative detection and biomarker discovery through proteomic and transcriptomic analysis. We have identified 904 proteins, among which 228 proteins are discovered, 426 proteins are detected from exosomes of dry eye disease, and demonstrate CALML5, KRT6A, and S100P for the classification of dry eye disease. We have also investigated 484 miRNAs in tear exosomes and show miR-145-5p, miR-214-3p, miR-218-5p, and miR-9-5p are dysregulated during diabetic retinopathy development. We believe iTEARS can be used for improving molecular diagnostics via tears to identify ocular disorders, systemic diseases, and numerous other neurodegenerative diseases and cancer."
Discovering the Secret of Diseases by Incorporated Tear Exosomes Analysis via Rapid-Isolation System: iTEARS (open access)
No comments:
Post a Comment